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CRIMIC CR71057

Gene GCC185

CG3532

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	:12010772..12015059	87A5-87A5

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TTCCTTCGATAGTTGAACAACACC	CTTTCTTCAATCTACAACGCGC

Images

Construct Design Sequences

sgRNA sequence	TCTTCGCTCGGATTGATTAAGGG
sgRNA2 sequence	TGTTCGCTAGTTGTTATTATTGG

 

left_200bp	right_200bp
CTTTTTATGCAACAGTGATAATTAAATCCTTAAAAAGCACATATTTTTAAGACAATATTATTCTTTCATTTAATTTTAATTAGTAATTTTTCCGTTTTTGCGTCACCTTCTTAAAAAAAGACGCTCATCACTAATTGGGCGGTGATGACAGCCCTATCAGCTGTTAGTGTGGCTGGAGTGTTTTCTTCGCTCGGATTGAT	ATAACAACTAGCGAACATCATAGGATATATAATATATAAATATTCGTCTTGTTGGATGCGGATAGTCGTCAGTCGTTGGGCAGTTGCATAGCATTCATTCTGTAAATCTTGTACAATTTCCCTTTTATATATAATGTATTTTAAAATTGCTCGTTGCTGTTTGGCACATCGTACTTTGTACACTCTTGTAATTTGTCCAC

 

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