CR71059

CRIMIC CR71059

Gene Gbeta13F

CG10545

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	:15860115..15866175	13F16-13F1

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
multi	OK	CGTAAGTACACCCACAACTGACC	GACTCTTACTCTTCTGACTATCAGCG

Images

Construct Design Sequences

sgRNA sequence	TGAAGACAGCGGCGTCCTTATGG
sgRNA2 sequence	TTAGATCACAATGTAGATGATGG

left_200bp	right_200bp
CTACTTCCCCATATGTATAGCACCATCTAACAAGAGCGAATCGAATCGAATCAACTTGGACGACCGACCGACCGACCGCACAAAGAAGGATCTATTTGGAAAGCCGGCTAGCCGATTCCCGCATCCGATTCCGAATACGAATACGTATCCGTATCCGAATCCGAATTTGAAACCGAATCCTCAACCAAATCATCCCATAA	TCTACATTGTGATCTAATGTATATGATTCGGTGTTACCGTCTCTGTGGTAAATATTAAATGAGTGCCGGACGTTTTGAATATGTGGGCGCAGCTAGTTGACTAGAACGTAGACCACAACAACAAAGAACTTTATATACAACATTATTTATATTGCTATATACTTATTAACTAAAATTATACAAAAAAACAAAATAAAACC