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CRIMIC CR71060

Gene Torsin

CG3024

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	:4648592..4650032	4C11-4C11		Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	ATCGATAACAGCGGCGG	GGCATACGATACTCAAGGAGAACG

Images

Construct Design Sequences

sgRNA sequence	TCCGCGGTGGAATGTATAGATGG
sgRNA2 sequence	CACAGCTTGTTCGCCATTTCGGG

 

left_200bp	right_200bp
GACTGAAAATGGGGCCTGAATAAACGTTTTCTTTGGCGCCATATGCGCAGAATTGCGGCATACTCAATTAACCAAAACATACCACTGACTGACTTACAATAAAAATCTGTTGAATGCCACTGCTACACAACTATCGATAGAAACGGTTGCCGGCAAGTGCTGCAATCTAGTCTTATGCTGCCTATCGCTTTTGCCCATCT	TTCGGGCTGCAAGACGCTGGAGAAGAAGGTGGCCATGGCCATTTACTGAGAAAGGACCGACCGACTCCACATTTTAATGCTTAGTTTGGGTTTCGGTTTGTATTTAAAAAACTATTTTTTCGGAATAAAGAAAACAAATTTTAACTACCAGGGATTTAGCCACTTGAGGCGGGCATCTTTGCGCAGCAGATCGGTATCCA

 

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