CR71061

CRIMIC CR71061

Gene Srp19

CG4457

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	:7347310..7348034	65F5-65F5		Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GAGGTATCATCGATATGACAAGAGATG	CGTGACTGACAGTTGATTCTAGC

Images

Construct Design Sequences

sgRNA sequence	TTTCAATTGGGTTGCTTGCGTGG
sgRNA2 sequence	AGCGCCGCTAATGACTTCAGTGG

left_200bp	right_200bp
CGCCAACTTCTATTAAACCAGATAAGCCATAAATTAAAATTACCTAAAATAATTTATTTCATGTACTCTTTTAATTTAAGTCGCTTATAAATAACCTCTTTTTTAGTAGCCAGAAAAAGCCATATAATTATTGGCTTTCTCGCTAAATATCGATTACTCCACCAGCTGCATGTGCCATCGCTATTTCAATTGGGTTGCTT	CAGTGGCAACTCGTTGTTTGGTTTCTTTTGTAATAAACGCTGTAAACAAAGATTTATGTGTGTTCTGGGCCAAGTTCAAGTGCGGGAATTCGGCCTTGGCACGCCGAAGTGTGCCAAAAAAAACCGGCACAAAGAACTCAATTAGAGAACCCAATTGGGAAATGTCTCAAGATCACACGAAGTCAGCATAAATTCCGGCG