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CRIMIC CR71063

Gene Tim8

CG1728

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	:11368445..11369283	10B6-10B6

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GGCAGGAGGAGGATGCTTAAG	GGATTACAACACAATGCATTTTAAACG

Images

Construct Design Sequences

sgRNA sequence	ACAAATTGACCGGGCTCGTCCGG
sgRNA2 sequence	ATTTAACAGTAGGACAGTGACGG

 

left_200bp	right_200bp
AATGTACTGTTATTTGCTCTATGGCGTCTATATTCTTGCCATTTAAATATGACAACAGTGCAAACAGTGCAATATGCACGGCATTTCCAGTATCGAATAGCTTTGCTATCGAATACACATCGATTTTTGTAATTCCACAGTTCACAGCTGTCAGTGTCGTTTATATAACCTCAAACCTTAGAATATATGAAATTCCGGAC	CTGTCCTACTGTTAAATCTATTTTTAAACAAAAAATTATCATGTAAGCACATTTTTTTCCGCGCTGCGTTTCGCCGCCGACATCCAAAGTATGTGGCCCGTAGTCTGCACCCAGCCCGAAATTGAAATCCGTGATTCGAGTTCCGGAATTAATATTTAATTCGGTAGCGCAACTCAATTGTTTGGTCGGCCCTACGTTAG

 

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