CR71064

CRIMIC CR71064

Gene Roc1a

CG16982

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	:647304..648597	1B13-1B13		Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CTCCGATGTGCAGTAAATACCATC	GACTAGCTAGTGGATTTCGCGG

Images

Construct Design Sequences

sgRNA sequence	ATCCTCGTCGACTTCCATGGTGG
sgRNA2 sequence	ACTTCCCGCAAGCTAGATGATGG

left_200bp	right_200bp
CATCGACGCCATCGATGTTGGCATCGATGTTTCGCCACCTTTACGAGAAAAAGCGGCGGTCACACTGCTTGAGCCTCACTGCGAGTGGTTTTCTATTAAAACGAAAAGGATAGGGACAGAAAAGCCAAAACTACGTTAGTTGTGAAAATTGCATTTGTGTTGCGAGAGTTTTGTCAGGATAAATAGGTGTATTTCCACCA	TGATGGAGGCCTGCTTCATCTGGACATTTGAATACCTTTTTAGATCTTAAGCTAGTGGGGCGATTCGAGTGAACTGGTAGCCTTTAGGAGCACAGTTACCGATTTCCGAGACTCCCCGGGAAGCGCACCTTTTTGTAACGCGTTAAATAAAAGCTGTAGAAGATGGAGAAGACTAGACGGATGTTTCTACCTTTCACCCT