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CRIMIC CR71065

Gene Ide

CG5517

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p0	:20371672..20375896	77B6-77B6

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GGAGGGCATGGTATTGGAGAAG	CAAAGCCGTTTTGCGTGTTC

Images

Construct Design Sequences

sgRNA sequence	TGTGGCTACGGATCAATCAGAGG
sgRNA2 sequence

 

left_200bp	right_200bp
CGAAATAGAGAACAAAAACGTCAAGGTTCCAGGTTGGCCACGCCACCCCTACGCCGAGGAGCGCTATGGACAAAAGGTGAAAATAGTGCCTATCAAAGACATCCGATCGCTGACGATAAGCTTTACCACAGACGATTTGACGCAGTTCTACAAGTCAGGCGTAAGTTTAAGGTACCACTGCGCTGTGGCTACGGATCAAT	CAGAGGATTCCTTTCTGGCCAGCCCACTGAACCTCTTGTTTAATTATAGCCTGATAACTATTTGACCCACCTCATCGGTCACGAGGGCAAGGGCAGCATTTTATCCGAGCTGCGACGTCTGGGCTGGTGCAATGAGTAAGTTCATCTAGACTCCCGATATCATAGTTACCATCTATCTATTCCTTTTGCAGTCTTATGGC

 

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