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CRIMIC CR71067

Gene mfas

CG3359

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p2	:11984862..11993315	87A4-87A4

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CTTTTTCAGCGATTGTTTCGGC	GCGAACAGATTGCGATGCG

Images

Construct Design Sequences

sgRNA sequence	GCATATGAATTATTGGGTCCCGG
sgRNA2 sequence

 

left_200bp	right_200bp
TATTTTCATTTGATTCTAAAAAGAGTTCCACTGACAGGCGCTTAGTATGCGGGTCAGGTAAATGCTGCGCAAAAAACCTAAACAATGAGCACGATCAGCATATATACTACATACAGATAGATACATATATTTAGTTTGGTGAGGATAGAGTTGAGCTTCTTTTTATGCGAGGTAACAATGGAGGCATATGAATTATTGGG	TCCCGGTTCCGTTTCGATTCGTTCCGCTCACCAATGGAATTTGCATGAAGGGAACACACACAGACACACACACACACACACACATATGGGCGCACAGTAGCCAGCTAATACTGTATACACTAACCACATCACTCTACACCACGTCCACTCCTACGCCCACTCCCACCGTTCTGCAGCTTTATGCCACATTTAACCCACCC

 

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