CR71070

CRIMIC CR71070

Gene wit

CG10776

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	:4064152..4071717	64A5-64A5		Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	no band	GGGTCGACTTGATATAGACGGAG	CACTACACGCTAGGATGATTGGTG

Images

Construct Design Sequences

sgRNA sequence	CTTTGGATGCGTATGACTGTCGG
sgRNA2 sequence	GTTCGATGAAGGATGAGATATGG

left_200bp	right_200bp
CTATTGATACGCCAACCGAATGTGAGATTTTTGTGGTGGGGACCAGAGGTTGGAAAGGGTGCGAGTGTGGCCCGTCTATTGATAATTCCTGTGTACATGTTTCTTATTCAGAAAAGACAGAACAATATTTATAAACTGATGCGTTCTTTCAGCTCCTTTTGGTTGTTCAAAATGCCGTAACATTATCGAGGAACCCGACA	CTCATCCTTCATCGAACACATATTCAACGATAGCCATAGAGCAGAAGACTTGAATCGCTTCCTGGAAAAGTCTTCTCACAAATCAGAAGGATAAAGGAGTATTTTGTTTTCTACTCAAAATTCTCTTCGATTTCAAATCAAAGAAAGAAAAAAATCGTTATTATTTATGAGGAAACATAGATACTGTCACAGAGATCACA