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CRIMIC CR71072

Gene l(2)37Cb

CG10689

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	:19123998..19126976	37C1-37C1

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
multi	OK	GCTGTTAGGGGAACAGTGATG	TGAAAGCCGACTGGACTTGG

Images

Construct Design Sequences

sgRNA sequence	TCGAATTACCTATCGCTAGCCGG
sgRNA2 sequence	TAGTACGTTTCGATATATGGCGG

 

left_200bp	right_200bp
GTGTACCCATAAGTCCTTTAAAACTAGTTATTGCCACAAATAAGCCTATAATATAAGAAACATAATTTGTAATAAAATAAATATAGAATATTATATATATATATAATATATTTTTAGCAATTTGATTATTATTGTTTGCTCACCCAGTGCTGAAAATAGCTAGCTTGGCAGCACTGCATGTTATCGAATTACCTATCGCT	TGGCGGCCCCACCGACAGTTGTATTATCGATAACAGGCCGTGTGGTTGTATTTCGTGTTTTGCATTTCAAAGAGCAAAATTGCACAATAATTTAATTTAAGTGCAACCAAAAAGCTTTAACAAGTAAGTCTATGAGCGAGACGAGCTAACTGCGTTGGAAGACCAGGAAGAACAGGGCATAAAAACACACAAACAGCACA

 

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