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CRIMIC CR71076

Gene tex

CG9615

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p1	:8307473..8308645	84F10-84F1		Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GAACAAGATTCGCGTGGAGG	CAAAGCCATAAAAGCGAGCC

Images

Construct Design Sequences

sgRNA sequence	AATATGCCTCTTTCGTTTCCAGG
sgRNA2 sequence	CATCAGATGCGCCTTAAACTAGG

 

left_200bp	right_200bp
ACGAGATATCGTGGAACAACACCAACGACCTGTTCTTCCTAACCAACGGACTCGGCTGCATGCATATCCTCAGCTATCCCAGTCTCGAGCACCAGATGACCCTGAAGGCCCATCCCGCCAACTGCATTTGCATTGAGTTCGGACCCACGGGTAGGCAGCCATTGCAATCTCATATCATATCTAAATATGCCTCTTTCGTT	TTAAGGCGCATCTGATGGTAAAGACCACCCTGTTTGCTTTCCAATTACCTTTTCGAGTGTGACTTTATCGACGTACCTGACCGTCGATGATGCACTTTCGTGTTAACTGGGTCACGTTGCTAGCCGAGCGAGCATTCTAGGTTATCAATTGCAGGGCTTCGCCGGCGGACGTGTTCGTGGCAGCCATTTCGGTTTTATCA

 

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