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CRIMIC CR71077

Gene thoc5

CG2980

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	:23954091..23956404	60A13-60A1

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GGGTACTACTAGCTTTATGAGATTGG	GACGATGGGCTGATCGTCAAG

Images

Construct Design Sequences

sgRNA sequence	TTAACAAAACTGATATAATGTGG
sgRNA2 sequence	TACAACACAATGCGCCAATGCGG

 

left_200bp	right_200bp
GAGCAATCGCAGCATTGTGTTATTTCAAGTTTGGAGAGATTAACTTAAGCTTACAGCAAATATTAAAGGATATATTTTGTTTTTTAACTATATTTACAAAGCCGCTACCAAGTGTGACCGCAGCAGTGGGGTATGCGCATATACTAAAATCATTTTGAAAAGTACCGGCGAATGTTATCGACTCGATAGCCTGGCCACAT	ATGCGGTTACTAAACGTTCTCACAGTTGCGTTTATTACGATTAGTAATACAATAAATAAGTAACATTTAAAGCTAGCTAGCTACGACTTCATTGGCAGTTGATCTTCTGGGAAGAGGTCGCTGCCAAGGATCCGGGAGGTAGTGCCGTCGCAGAAGGGTTCTCAACCGCCTCCCGACTCTCGTCCGGCATCACTTTGTCC

 

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