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CRIMIC CR71078

Gene thoc6

CG5632

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	:12190696..12193688	69A1-69A1

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GAGGAAATCGAATTGCAATCTCCG	GAATATCAAGTGCTGCGTATTTCACG

Images

Construct Design Sequences

sgRNA sequence	CTGTTCTTGGTATATTGCTCAGG
sgRNA2 sequence	ACATCTCAATCTTGCTGTCGAGG

 

left_200bp	right_200bp
AATTACAATTGGTTTTCAATTGTATTTCCTCAAAATCAAAAGAATGTCGAATGATTGAAAACAGGTTATTTCGTAAATTCAAAATCCACGTTACGATTTCTAACGGTTAACGTAATTGAACGTAACGTGAATGCTACGTAATATACTAACGCACTTTTAGGTATATTTTTCAAAAATAATATACTGTTCTTGGTATATTG	CAGCAAGATTGAGATGTATGGGAATGTGGAGGGCGAGGAAAACGACCCAGAAGAAGAAGACGAACTTATTGAAAAACCTCTGACAGTTGAGGCGGCATAAAGGTGTAATAAATTTGTTCTTTAATTACATTATTATACTGTTTACATTTTTTAAATGCATTTATTCCTTAGGGAAACCGAGCATATTCCTCGTTTTTACA

 

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