CR71078

CRIMIC CR71078

Gene thoc6

CG5632

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	:12190696..12193688	69A1-69A1		Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GAGGAAATCGAATTGCAATCTCCG	GAATATCAAGTGCTGCGTATTTCACG

Images

Construct Design Sequences

sgRNA sequence	CTGTTCTTGGTATATTGCTCAGG
sgRNA2 sequence	ACATCTCAATCTTGCTGTCGAGG

left_200bp	right_200bp
AATTACAATTGGTTTTCAATTGTATTTCCTCAAAATCAAAAGAATGTCGAATGATTGAAAACAGGTTATTTCGTAAATTCAAAATCCACGTTACGATTTCTAACGGTTAACGTAATTGAACGTAACGTGAATGCTACGTAATATACTAACGCACTTTTAGGTATATTTTTCAAAAATAATATACTGTTCTTGGTATATTG	CAGCAAGATTGAGATGTATGGGAATGTGGAGGGCGAGGAAAACGACCCAGAAGAAGAAGACGAACTTATTGAAAAACCTCTGACAGTTGAGGCGGCATAAAGGTGTAATAAATTTGTTCTTTAATTACATTATTATACTGTTTACATTTTTTAAATGCATTTATTCCTTAGGGAAACCGAGCATATTCCTCGTTTTTACA