CR71080

CRIMIC CR71080

Gene Son

CG8273

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	:9578042..9581225	85D25-85D2		Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GTTGGAGTTGTAAGCTTATGAACCAG	AGGGAAGGAAATACACTTCAAATACG

Images

Construct Design Sequences

sgRNA sequence	CATTGCTCTCTCGCTGGGCGCGG
sgRNA2 sequence	TTCAACGTTCGATCACTAGCTGG

left_200bp	right_200bp
GGTACTATTACACAACTCTAGCAAAGTATCGATATCTTTGAGCCCTGCGAACTGTAGATACCATTACGAGTGTCATCGCTATTATTGAAACTTCTGTCCAAGCAAGACGTGCAGAAAAACGCTTAATCGCGAACAGCGACAAATTCGCTGATCTGTTGCGGTAATCTATTAGCATGCATAGACACTAGGACGATCCGCGC	AGTGATCGAACGTTGAATAGGCTAATTTCTAGATAGTACACATAATAGAATCCCCACACTGTACATAAAATTAGCCCTATATTTAAGTTGTAAAATCTTGTTCGTCTCCTTTGGAGCGATGACAAATATAGTGCTAACATCAAAAATGATTGTTAGTTTGACTAAAATTACTGATATTTGATGTACCCCTAACGTTATCA