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CRIMIC CR71081

Gene sbr

CG1664

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p2	:10832752..10847092	9F4-9F5

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GAGAAACGCTTACCGAACCTCAAG	GCTGCATACGATCTGTCCTG

Images

Construct Design Sequences

sgRNA sequence	CAAATCCCACGTCGAATATGCGG
sgRNA2 sequence

 

left_200bp	right_200bp
TCAATAACTAACTGACGACCATTACCTTTTCCAGATACCATCTTTGGCCCACCTTGTAGTGCTTCGCAATCTGTCCATCTTGGAACTCGTTTTAAAGAACAATCCCTGCCGTTCCCGCTACAAGGATTCCCAGCAGTTTATCAGGTATACTATGGTGTGATTCGTTGATCTTCTAACTTTTCTAGTGGATGCTGCCGCAT	ATTCGACGTGGGATTTGTCGCGTCTGGAGTTACAGAGGAATTAGGATTACCACATTTCCATTGTTCTGTTGTCTCCATTCCTTTTCTTTGAAGCAAATGCGCTGCAGAATTGGGGACCAAGGTCAGGTGTTGCCTTCGTCCAAAGTTTAAAGCAGCTGAGCAGGCAAAGGCATTTGGGAAATATTTTCCTTATCAGCTGT

 

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