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CRIMIC CR71082

Gene nxf2

CG4118

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p2	:16576859..16580306	CR71082

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GTAAGCCTGGGCAATCCGAG	CAATGTTTGTTTCCCACTGCCC

Images

Construct Design Sequences

sgRNA sequence	TCGGCTGACTTAATCTGGAAAGG
sgRNA2 sequence	TATAACGAAAGAGGAAACAGAGG

 

left_200bp	right_200bp
GATGAACGTGTGCCAGGTAATACACAATGATGACAATGAACGCTTTCGGCTGAACGGATTTATATTGTCTAACAACCGCATTCGGGATATACGGCCCTTGACGCTACTCGCCAATGTGGACTACGCCCTGCTCGACTTAAGGGGTAACAAGGCGAGTTGACTCCTCCTGGTTATATGAGCCCTATATAAAAGTTCCTTTC	TTTCCTCTTTCGTTATAAATACTGAACCTGTGTTGCGAATTCGACATCCCAATAAAACTACCTTGAATTACCAATAAAAAGTAATTTAGATGGCCGAAAACATTTATTTGATAATTAAGAGTTAATCACTTGCAAAGAGAAGTCTCTTTTCTACACATGTACGGGAGCTATATATCCTCGACTTCGAATCCGTAGCTGCG

 

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