CR71083

CRIMIC CR71083

Gene CG7483

CG7483

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	:8313208..8314864	84F11-84F1		Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CTGCGCAGGAGAAGAAGTAGC	CCAGACGAGCGTAGATTGTAGTGT

Images

Construct Design Sequences

sgRNA sequence	GTTCACGATTTGTTCGCGTGCGG
sgRNA2 sequence	GATCAAGTCAGCCACGTTCATGG

left_200bp	right_200bp
CGATATCAGTGTTGCCACTTGTCGCAAAATAAGTGAAAACATATCGATATCAAATTAAATCGACGCCGATTCAATAATCGATTGTTGCTAAGCAATTGATAGGCCACCCCTAGTAAGCGGACCGCATTTTTTTCTGTGAAAGTCAGAAAAATTAGCCGAAAAAGACTGTAAATATTTATTAATATCAGCCGAAACCGCAC	ACGTGGCTGACTTGATCTAAACTAATATTCTATCATTACTTTTATGTAACGCACTTTTCCTAGCCGTAACAACAAACGAATGAAGTAAATACATTTGAAGAGTATTTGAATTTCAATAGACAACCAAAATCTGTATAATAATAATAAATAACACGTAAGAATTTAATACAGAAACCAGTTTGCTTTTCAAATGGGTTAGG