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CRIMIC CR71086

Gene CG1418

CG1418

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	:9963835..9965067	46C7-46C8		Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TGCATTCAGCGCTTTGGA	ACAGAGTCCAATGACTTGACCAC

Images

Construct Design Sequences

sgRNA sequence	ATGCTGCGGCGTTTGTAAAGCGG
sgRNA2 sequence	AAGACCTAGGGTTTAGCTTCTGG

 

left_200bp	right_200bp
CGCACTCGCAACTCTGGCCACACCGCACGTCACGGCAACGGCAATCCTAGAACTATCTGCCACATCAGATTTTTCAAACTTTGTATACTGTTTCTCAAAAGAAAACGATGTCATAGCAAATTAAGAGTGAATCGTAAAATCAACATCGAAAGTGTTGCCGCCTCCATGTAGAAGTGACCCCGCTAAAGTCCTCGCCGCTT	GCTAAACCCTAGGTCTTCTGGGATCGTGGCAACCAGGGACGAGGAAAGGGGCTAAACATATTCGCCACTATGGGGCCTTGCAAGTCGACACAATATGTTGTACACCTATTTATAACGAAATCTAGTTAAGCCGCAGACTTTAAATAATCGAAATACACACTATGACAGATTCATACGACTATATTCTTTTTGGCAAATTA

 

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