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CRIMIC CR71087

Gene Irp-1A

CG4900

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	3R:22721320..22724587	94C3-94C3

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GCATGTAGGAAGGGAAAAGCG	AGAAAAGTCCACGGGACGGACAAA

Images

Construct Design Sequences

sgRNA sequence	GATATAGAGATGAGCGCGTGAGG
sgRNA2 sequence	AAGTCGCCTAGAAATGCATATGG

 

left_200bp	right_200bp
GCAATCAATCCAATATTATATCCAATATTATTTTCGAATCACAAAAATGATAAATTATTCCACTATAATTTACAAATGTTTCATTTTAAGTAATAAATAAATATTTTTTACTGTGTACCGGTTTGTTTTCATGTTCAGTTATCGATAATATAATGTTTGCGTTAGTGATGAGCAAGTTTTGCCTGGTAGCGGTTCCTCAC	GCATTTCTAGGCGACTTTTAATTGTTATTATGTGCTGGGAAATAAAATTTGACATTCATCTGTGAGATGATAATTATTAAAAAAAAAATATTTCAAATATTTTATAATATTTTAAATTGCGCGCTCTGGATGTTATCGATAGCAGAAATGCCGATGGCCAAACCAGTCCTGCTAAAAATGCCTAACGCCTGCTGGTGAAT

 

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