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CRIMIC CR71088

Gene Irp-1B

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p1	3R:10408618..10412869	86B4-86B4

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CGACTACCATATACCCTGTCCC	CCCAAATCAGCAGATCTGCGTAGT

Images

Construct Design Sequences

sgRNA sequence	AATTAAGCACCTGTTGAAGTTGG
sgRNA2 sequence

 

left_200bp	right_200bp
TCCCTTCGCCCAGTTCGAGAAAACTTTCTCCCAGGCCGGCACAACATACAAGTACTTTGACCTCGCCTCCATCGATAGTAAATATGGTAAGTGGATGTGGTCTTAAGAAGGCTTTAAAAGTGGGCGTGTCTGTCTACGTGGTCGCAATAGACTCGGCTAAACATGCATATCAAAAATGGGATTAATTAAGCACCTGTTGA	AGTTGGAGTTTGACCCCTGAATTGAAAAAGAAGCGTAGGCGAACAGCTCAATTTGCAAGTCAGGCAATGTCAAGTAGAAGGTGCTCCCCGAGCATTAAGTGGATTTTCTATAGTTTTCGATGGGCGGGCTTGTCTTGCGCCGAAAGCGAAAGTTATCACCGCCAGATTGCCTCATAATGGGGCTCGTCTGGCGGTGATAA

 

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