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CRIMIC CR71089

Gene mAcon1

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p0	2L:21168570..21173185	39A7-39A7

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TTCGCACAGCGTTTTCTAACC	CACGACTGTATGTACATAGACGC

Images

Construct Design Sequences

sgRNA sequence	CCTCTAGAGCATATATCTTGTGG
sgRNA2 sequence

 

left_200bp	right_200bp
AGTTCGCAGTGTTTGTGATTCTACAGGCCCTGCATCCCAAACATGTATACTGAATACGCAGCAGTGAACCATGCGAAATCGTTAGCTCTCAACAATTAACAGCGATTCGTGGGGGTTCGATGGTTGGCATAGTTGCATTTACATAATCGCCGGGGTCCAGTTATTTAACCAGGAGGCGAACACCCTCTAGAGCATATATC	TTGTGGCGGAGGACCGTCTTATCTGTGACAGGTTTACTTACACTTTGATAATGCCAGCACTAAATATAGCTTAAAAAAGTTGCGCCGTTTAACTTGAGTATCACGCGCATTGGAGGAGTTTTGCCTTTCAAGTGGGGTTTTTCCACGTCAGCTTCGAGAAAGGTTTCACTGTACTCTGCCCATAAGTTAACGACCTATTT

 

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