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CRIMIC CR71098

Gene aurA

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	3R:11963124..11964826	87A3-87A3

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	ATCTTCCGATAGTCTATCGTCCG	CCCATTTCGCTAGAGATTAGGTAGC

Images

Construct Design Sequences

sgRNA sequence	GGTAAAACATTCACGACTCCCGG
sgRNA2 sequence	GTGTTCACTGCGTGTGCGCCAGG

 

left_200bp	right_200bp
ATGCCAAAGTTTAGAGCACTATTCAATAATTCTCTTGATTTCAAATTGAAATAATACACAATATAACATTTTCTAACACTACAAAGTCACGATATTCTTCcACCAACCGATAGTATCGCACACTTGCCATTCGCCTCATCACGCACACGCCCGCTTCACAATTCAAACGAACGGCATTTTATTTTCACAGGATCCCGGGA	GCACACGCAGTGAACACATTCTTGTTTAATTTTCAGTTAATTCCTTTTAATTACATTTTTAGTTTAGGGCTCCCCGCGTAATTTAATTATGGCATCATAAATTTTCATTTATATAGATACGAGAAATTAACATTTATCAATTAAGTTTATTATTATCATTAGCAATAAAGTTTGTGTGTGTTTTCGAATGCTAGCGTACA

 

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