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CRIMIC CR71099

Gene Mcr

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	2L:8074117..8082982	28E1-28E3

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GCCCTCATTAACGACTTGTTCGCA	GTGTGGCAGAAGATTCTTTGTTCAG

Images

Construct Design Sequences

sgRNA sequence	AGCAAGTGCCACATCATTGCTGg
sgRNA2 sequence	AATCTTAGCAAGTTGTTTCGAGG

 

left_200bp	right_200bp
TTCATACAAAGCTTACTATTTTTTATCGAAAAATCGAATAAACTCGAAAtTTgGTTTTGAAAACCATTGATACACCCTATTCGACAGGCATTAAAGAACAGGCCGATCtCCCAAATCAGAGGCCTCTATTTATAGTATTTGGGAGATGCAgTTATGCtCAGCGATTCTAATTCATTCACTTCTGACTTCTCCTCcCAGCA	AACAACTTGCTAAGATTCGTAATGTTTTTGTTCATTTATTGAATGTGTTTACTTTGTAGTTTTTTGTTTTGAGTTGTAGTTTTTTTGTTTGCCAACGAAATTGCATTCCGTCCAAAAATGCTGGTCAACATTTTGGTTTTATTTTTAGTTCTTTTCAGTTTTACTCCACTGACCCGCTGTAgAATcGAATGTACAAAACA

 

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