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CRIMIC CR71100

Gene CSN6

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	3R:22580773..22582033	94B5-94B5

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TGGCGCATAATTAAGCTAACAGG	CTGCACTCGCTCATCATTCCC

Images

Construct Design Sequences

sgRNA sequence	CTTTATTTACACGTAGTTTGCGG
sgRNA2 sequence	AATACACATAATCGTACGATGGG

 

left_200bp	right_200bp
TCGAGCCGGCAACGCCGACGTTCAGTTTAAAAAGGTTCGCGCCACTGCTTAGACTGCATAAGGTGTGGTCACACCATTCTGCTGTCCCGATAGATAGCGATAAGTAATATCGTACTACTGAGAGACCACCAATAACGATAACGACAGCTTATACCGGAGCTTGTCAAACAAAAAGAAGTAAAATTAAGTTTATACCGCAA	GATGGGTGAATGCGGTTTTATTTCAGCTGCCGCTGGGCAGGCTACATACGTATACGGGTGGCATCAAGCGATATCACTTTCTTCCTGAGCTTCCAGCTCCTCTTGCTTCGTAAATTCAATTCAGATGCACAGACAACATTTCCTAGACACTAAATTAGGTAATTTCGCAGCGGAATTAGTGGTTGTAGTCGTCCAGCTTG

 

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