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CRIMIC CR71101

Gene Cpsf100

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	3R:29034573..29037062	98F5-98F5

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TATGCAACCGATTGGGCATCCATG	GAACCCTCCTCATCCGAAGAG

Images

Construct Design Sequences

sgRNA sequence	CTGAACCGGCGAAGAACTCTTGG
sgRNA2 sequence	CTTAACAAGTAAAACATTAGGGG

 

left_200bp	right_200bp
TGAAGAAGGTTGTAGCCATATGTATGTATGGTTAATACATGAGAGACATAATCAAAGTTTTTAATTTATAACATTTAAAATGTCAATCTTTGTTTATTATCGGGTTGTACTGTTCGCTCAACAAGTACGTACTGTATAAACAAAGCAACAGCACTCTGGCAACGCCGTGACAGTTTCCCATCCCTGAACCGGCGAAGAAC	ATGTTTTACTTGTTAAGATTAGAAAGTTTTTATATAAGAGAATCAGTTGTACTTTATTGCCTACATAAATTGCAGCATGGGATCTTCTGCCCTTGGCCGCTTCTTCTTCCACTCCTCGATCTCGTCCTCTGTGGGCGCCTTCACATCATACATGCTGTTATAGGCTCGTTTGCGATCGTCCAGTTCCTCGGGAATATCTT

 

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