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CRIMIC CR71102

Gene CG1275

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p1	3L:2252744..2257423	62D4-62D4		Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CAACATGGATCCAGCGCTGATC	CTGCAGCGAGAAGACGATCAC

Images

Construct Design Sequences

sgRNA sequence	ACCATTTCGAAGCTAGTCAATGG
sgRNA2 sequence

 

left_200bp	right_200bp
TGTGCTCACCCAGCTGTGCGGCCTAACCATGATCGTCCTGGTGGCCACCTGGATTGGCCAGCACTTCGGCGGTCTGGCCGGCACCTCCAATCCCGGCGTGGAGTTCAACTGGCATCCGCTGTTCATGACCATTGGCTTCATTTACCTGTATGGCAACTGTGAGTAAATGCAGCGGAAGAGGTGCCACGCCACTCCCATTG	ACTAGCTTCGAAATGGTAATCCAACTCAATTCAATCCCACAGCCATTCTGATTTATCGTGGTTTCCGCACCACGCGCAAGAAGACTCTGAAGCTCACCCACGCCGGCATCCACATGGGTGCATTCATTCTGACGGTGATTGCCCTGAAGACGGTCTTCGATTCCCACAACTTGGCCAATCCGCCCATTCCCAACATGTAC

 

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