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CRIMIC CR71103

Gene PAPLA1

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p1	2L:8134247..8159638	28E7-28E9

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
long band	OK	GGAGACTGGTGAATGGCATCAGAA	ATAACAAATAGGTCTCATGGGTGC

Images

Construct Design Sequences

sgRNA sequence	CAATTTGTAATCATTCTTAGTGG
sgRNA2 sequence	GATCCAACAGAGCTCTCGTGTGG

 

left_200bp	right_200bp
TGCGTCCGAGAGTTGTCAAGAGGGATCTGGATGATTTCACCATCGAGCAAGGCGAATCGCAGCGAGTCGATCACCTGCTTTTCATGGTCCATGGCATTGGCTCAGCCTGCGATTTGAAAATGCGTTCCGTGGAGGAAGTGGGTGAGTTATATACATGAGTCTCTTAGagTTCTGaCAAATAACCAATTTGTAATCATTcT	GAGAGCTCTGTTGGATCCAACGATCCCACGTACCGTATACACCATAACTATATATAAATTTAGTACCAATTTATTTAGATATGTTATTTGTTAGCCGAATCATGCAAAGAATTTTCCATATGTGCAACTCCAACATGGTAAAAGGTATTATCAAAATAAGAAGTCTTTATCGTTTGAGTGACAAAACCGTAGCAAGTTCT

 

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