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CRIMIC CR71104

Gene DEF8

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	3L:12132146..12133834	68F5-68F5

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	AGAAGGAACAATAGAATAAAGGGCTTATTG	AAACGCTAACGGCACCAATCCCTT

Images

Construct Design Sequences

sgRNA sequence	CGACCAGGGATGCGAGCAATTGG
sgRNA2 sequence	TGATTTGATCAGTTATTCTGCGG

 

left_200bp	right_200bp
AAACCACGCTTGACATATTTCAGAATATGGTTTCAAATTGTATCATATTTTAAAAATATTAAAAGTTAAATTCAAAATATTTATAAATAATTAACCCTCACATTTTTTCTTTCACTGTATTAAGGTGCATTCCAGGGATGCGTACAACCACCTGCAGTTTGCCCCGTGAACGATAACTAGTTCGCTCATCTGCACCAATT	AATAACTGATCAAATCAGAGTTCATTGTGCTGTGTTGGTTTTTAATATGTTATCATATGTACATAAAATGTAACTATAAAATGTATAACTATATATGAACTTTTATTCTTAGATTTATCATAACTCTTTAGTTATCCTTTTTTCCATATTTTTCCCGCTTGTGGAATTTTCTGGGATTTATGCCATATGCCTTTAATCGA

 

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