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CRIMIC CR71107

Gene escl

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p2	2L:11990927..11992848	33B5-33B5

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GAGTGTCTGCAATGTTGTGGAG	ACATTAAAGATAGAGCCCACGCC

Images

Construct Design Sequences

sgRNA sequence	ATGGGAGCTGCAATGCATCCGGG
sgRNA2 sequence	GTATCGGATTTAGAAGGATAAGG

 

left_200bp	right_200bp
GAAGTTAATTCCGTGGATCGGGAGGACACCGCCAGTTTGTTCAGCACTACTACGACCACCACCAGAAGCAAATCGCCAAATACACGAAAATTGAACCGCCTGTGTCGGCGGATCAAGGCACCCAAAATGGTTCAGCCCTTGTACAAATATAGGTGTGATTTTTCCAGGCAGAACATAAGTCATAATGTTTATATCCCGGA	CCTTCTAAATCCGATACCATATTTATGTAACCATAGTTATTTTCAGTGTAATGTTTACCTTCAATATTAAGTATGTTCTTATGCGTAGCATTTATATACAATACTCATTGTATTGACAATTTATGTTAAATTATGTTAAATTTTTTAACATTAACCAAGATATTTATTCTATTCTATTACTTTTTTAATGATCAAATAGC

 

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