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CRIMIC CR71108

Gene Mnn1

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p1	2L:7056301..7063586	27D7-27D7

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CCGATGTTATCAATCTGTTCCGTC	GCTTCTCGACCACCGACAG

Images

Construct Design Sequences

sgRNA sequence	ACTGGTCACCCTTTGCTAATTGG
sgRNA2 sequence

 

left_200bp	right_200bp
CCTGATCTCACCCTGCTCTCCATTGTCGTTGGCTACATCGAGCTGTCTTTGACGACGGGAGAGGCTGCTGCGCAGGCCGCTCAAGCCGCCGCGGCCGCAGTCGCAGCAGGTGACATTAGTCAGGCGACGACAGGTGTGTACTGCACGCCGTTTATCACTCCATTTTACCTTTTACCGCTCGCATTTACCGTTAACCAATT	AGCAAAGGGTGACCAGTTAAAACGGAAGCGATTGCATACAAATTGGAAAAGTTTCTGCCTCCTCTTTGCTGGTTTACTTCAGAATTAAAAACTATTAGCAACTTGTTCACTTATAATTGCAGGTGGAAATGACATCATCATGGGCAACAGCGTCCCGTTTCCAGTTGTTACCCACGAACTGATCGCCGGGCTGTATAAGA

 

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