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CRIMIC CR71110

Gene eco

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	3L:7335442..7339340	65F5-65F5

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CTGCAAGCTGACTTGATCGC	GATCGCAAGTGCAGGTTGTG

Images

Construct Design Sequences

sgRNA sequence	ATCCAACCACCCCCAACACTAGG
sgRNA2 sequence	CCTCACCTACGACCAGTGATCGG

 

left_200bp	right_200bp
TAAAATGTGGTAAAAAAATTATAACTAACAGAAAATATTTACATTGCAATGTATTTGATTTTTATAAACATTGATGTAAAAAAAATTTAAAAACAATGTAATGCATTTAATATATATAAACATTGCAGTAAAAACACAGACGATCTGACAAGTGTTGGCAAACGACCGAAGAGACCCCGAACGATCCAACCACCCCCAAC	GATCGGGGAAATCTATTGAAGCTATTGAATAGCTGGATTGTTTTTGAGTTAGGATTGCTTCGAAACCTGTAATAGTTGATAAGCAAGTGCGATTATGTTACTAGCGGCACGAAGACGCGCAATTTATCATGTTTACAATGGTGGTTTAATATGCCTGTTTTTACGCAATGAACACGATATTTTCTTCGAGTCGCATTAAT

 

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