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CRIMIC CR71111

Gene Fit1

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	3L:4103564..410716	64A6-64A6

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	ACATCTGGCGAGATAAGGGC	CTTCTGGTGTCGGACACTAGTC

Images

Construct Design Sequences

sgRNA sequence	CAGAAGAGACGCCCGCGCATCGG
sgRNA2 sequence	GACGGGCGGCTGGTCGTAAACGG

 

left_200bp	right_200bp
AGGCCCCCCCTCTCCGCTCCCCGCCAGCCAACGACAACGACAAGAACAACAAAAACTTGGTTGCAAAAACGGTGCTGAACGTAAGTTGGATTACGTCATCGTTTCGAGGGCAAATTTAGACTCTGATTGTGCCATATATATACACTATACAAATATACCCGCAGTTATAGTTTCGCCTTTGGACAGAAGAGACGCCCGCG	AAACGGATTGGCGCAGTCTGGAGCATTGGACTGGAGTCTTGAGTGCCAAGCACAAACCTCGTCGCATGTTCTACAGTTTTATCTACATATATTTACCTAATGTAGGGTGCGTTGATTTATGGGTTGACTGGTAATCTGTGGACCACACCAAGCAACTTCTTCGTAACTCTAGTTGTTAGTTTCTCAATGTTTTTTTTCTG

 

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