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CRIMIC CR71114

Gene Golgin84

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	3R:24356070..34357938	95F15-95F1

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GATTACACTGCCCATGTTGAGTATTG	ATTAGCATCGGAGGCGATGCTGTCTA

Images

Construct Design Sequences

sgRNA sequence	CTTAGAAAAATATGTCATCCTGG
sgRNA2 sequence	AAAATTAGCACTACTAAGTTAGG

 

left_200bp	right_200bp
TTATTTCTAAAACCAATCCTTACATTTTCATATAAATATTTTTTTGATCAGAAAAAAGGTTTTTCAAAATTACGTTACTTTTAGAGTGACCTTAACTTTGGAGTTGACCATTCCGCAGTCGATAGCGATATGGGCACACAGTGTGATATTGTTTACGTGTTTTGCTCGAAGAGATTTGTTTTTCTTAGAAAAATATGTCA	GTTAGGATTAGAACGATTCCTCACAGTTCAAATATTGCATAAAGAATCCATCTACAGATGCTGTAAAATTGTTAAGTAAACGTGAATTAGTTTAACTTCAGTTTAACTCCAGTTGCTGGGCAGAGCTGATAGTTCTAGCTAAGCGGAAGAAAATAGAGAGTATACGCTTTTGGGACGATTCGCGTAAAACATGCGCTATA

 

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