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CRIMIC CR71115

Gene PolrMT

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	2L:1103254..1108373	21F1-21F1

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	multi	TCGTTGACAACGGTACAGCTAGTC	AAAGCAGGTGGAATCCACGATGTG

Images

Construct Design Sequences

sgRNA sequence	ACACACCGATTCCGACCAATTGG
sgRNA2 sequence	TAATGACTACGATTAAGCCTAGG

 

left_200bp	right_200bp
TACAAGAGTAGCCTTATAAGTAAATTCGAACTTTTGTAGTAACTGGCAAATAAATTCAAACTTTTGTTATTTGCAGTATGTTCTGCAGTATTTTCCTCTCGCCAGCCCTGGCATCGCAGTCAGCTGTTCTGCCCAGCAAGCAGTGCTGCCAACAACTGCTTGCGCCGCCACACGTGAAGTGCGGGAATAAACAACCAATT	CTTAATCGTAGTCATTAGTTTTAGTTAGCATACCAGTTAAATTTGTTGAACAATTCGGCTTTCGATACCAAAAAACACGAAGGAAGACAGAAGCTACACGAGGCGCCAAATGGATCGACGCCAGATGTGATTTTGTAACGATACTTACATTAAATTGATTAAAAAAAGCGCTTTTAAAGGACATGCTGTTACCAGTTTTT

 

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