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CRIMIC CR71116

Gene Prosbeta5

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	2R:10819978..10821178	47C1-47C1

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CGCATTGCAATTCGCCAC	GGAAACATTGCAAATGATCTCACAG

Images

Construct Design Sequences

sgRNA sequence	AGTACGGTCACTCTACAAGTAGG
sgRNA2 sequence	GTAGTGCAGCTCCATGCAGTCGG

 

left_200bp	right_200bp
TGCAGTATTTGGCCATTGATGGATTGATGGAACGCCCAGAGTTAATTTGGCTTCAAGCCAGGCCTAAAAAGAGACTAAAAGAAAGCCAATCACAGGCAAAATTGGCAACAGGTTTCGAGCTGGGTTGCCGTACCTGCGACAGCTAACGGTGAAGCCGATAGTCTGCATTATTGCTCCAACAGAAGTACGGTCACTCTACA	GCATGGAGCTGCACTACATGTACCAGGAGCAGTTGAAGCAGCAGGCCGCTAAGTAGAGTTTTGATTAAGGGAATATAAGATATTCAGTTTGTATACGTTTGAATTTCTTGGAATAAATAAACGTGCTAGAGACTATGTTTTGTGTATAATTGATTGGGCTAATTTGTTTTGCTTTTCATAGGTTTATTACTATAAATGTA

 

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