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CRIMIC CR71117

Gene Prosbeta5R1

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	2R:23308654..23309816	59D6-59D6

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CCACAGAAGGTCCATTGGATTC	GGCATTTGTATGTATGCAGCTCTTG

Images

Construct Design Sequences

sgRNA sequence	TAAACGAGCTCGAATGATTGTGG
sgRNA2 sequence	AGACAATTTGCAGACTAAGCTGG

 

left_200bp	right_200bp
TCACCCACTAACCATGAAATGCCAATGGAATCGGAGACTGCGAATTGCCGCTGCGGATTGTCCAGTGGAGCGAAACTTTCAAGCGGAATGCGATATTTTGATGGGAACAAGATGTCCAGCTTTCTGATCTTGTGATCTTGTTTTGATTAAAGTACATTTTTGATACACTTGTAAGATGCCTTAGAGACTTAACTCCACAA	AGCTGGCAACGGTATGAAAATTTGGAAATTATTGGAGCAACATTAGCAAAAATTTCTGTGGAAGTAAAACTTCTGTCGTGCAAAACGTATAAAGTGTTAGTGTTTATGGTAATGGGAATTTGTGGCAGTGAAGAAGTCCGGAATTTATCGTGAGAATAGTTTTCCGAACCGTATGCTGGATTGTTCTATCTAATCCTCTT

 

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