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CRIMIC CR71118

Gene Prosbeta1

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p0	2R:16013805..16014787	52D13-52D1

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GTTGTCAGTGCGAAAGGTGTATG	CACTCTCGCCGCAGTAGTTATC

Images

Construct Design Sequences

sgRNA sequence	ATGGTGGTCTGCAAATAGGTAGG
sgRNA2 sequence	CTGATCGATCTTACTTGGGTAGG

 

left_200bp	right_200bp
TCTGGTAACACTTATCCAAAACAAGCCAGTCATTTCGTGTTCGTGCGGCGTTTCAACAAATATCAATTGTAAATTGAATTGTAACAAATTTAAAGATCAATATGCAGCCCGACTTTGATTTCACCGACACACCGGTCAGCACTGGTGTAAGTATGAAGAGCATCAGCCACATAACTTCGTACTAAGAGCACAATCCTACC	CAAGTAAGATCGATCAGAGAAAGAGTTGCAAACTCGTCTCATGCTAATAAACCTGGTTTTTGTAATATGCATACTTTAATTTGTATTTTGGCTCTTATGGCAGTACAAAAGTTAGGTCTAGGGGCTAAAATGGTAATAAATAATCGAAAACCTAAACCTAAGATGCATAGTCATACAGATAGTGCTGTTCCTGACACTGG

 

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