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CRIMIC CR71119

Gene Rpt5

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	3R:23752876..23754623	95B1-95B1

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
multi	multi	CGTTCTGTGTAAAGCCTGCC	AAACCTCATCAACCACTGCCACGT

Images

Construct Design Sequences

sgRNA sequence	ATCAACAATCGTATTAGACATGG
sgRNA2 sequence	GCTCTTAAAAGTAACTAGAATGG

 

left_200bp	right_200bp
CTGTGTTATCGCTGTCACTATCGATAAGCGCCGGCGATGGCGCTCTGTTGTCAACACTGACTTTTTCAGTGTTGCTTACTTGCCGCTGAAAAACAAGAAATTTACAAGTCATCGACAGAGTGTTTGCCCAAAAAGATCGGCAATAATAGCATTTTAACAAAGAATTACATTCAGCAACGGCTAATCAACAATCGTATTAG	GAATGGGAGACGTACTGAAATCAATACCCAACCTTTTTAAAATAAACAAAAATATATATCAAATGTCGAGCGATTCATTGGTTTATCAGTACAACATTTACATTCTGAGTACGATATTTTTTAAATATCTAAAAAGGTCGGTTTTAGAATTAATAAAAAATATTACATCGGGCGAAATTAGATGATAAATATTTACACGC

 

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