CR71122

CRIMIC CR71122

Gene Arr2

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	3L:8647261..8648936	66D9-66D9		Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GTTCTATCTTATTGTCGTGTTGGACAAG	CCCTTAGAATTCCTGCCAGCAGAT

Images

Construct Design Sequences

sgRNA sequence	AGAAATCCACTAATCCACAATGG
sgRNA2 sequence	TTTGTTGCAGCAGTCTCTCGTGG

left_200bp	right_200bp
TGCATAACGAGCAAATCCAGTTAGTGGCCAGAATGAAGACCCACGGACAGGGCCAGGGCAAAGGTATATAAGCAGGTTGTACGGCACTTGGGTTTCGCATTTTCCTCCGCCGAACGGAAAACTCTATAACGCGCATCGCCCGACTTAGCCTCGAATTGCCCAGTTGCATTTGCCAGGAATCAAAGAAATCCACTAATCCA	TCGTGGAGGCCACAATCTTTGTATTATAATCGTATCTATAATGTAACTTTGGTGGATCTGGAGTTTGTACCCGTACATATGCATGTTGATGAGTTGAACAGACCTCGTGTCGCTATATTATAAATGTGTTATGGCTATCAAAAGCACTTACCTTTTCTCTCGTTTGCGATGGAGTATCCAAAAGTGATTCGGTAAGGATT