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CRIMIC CR71123

Gene tst

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	3R:23768399..23772859	95B1-95B1

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GTCGAAGTGTTCCGTCGCAAGTAA	CATGACTTTCAGTCGGGTGC

Images

Construct Design Sequences

sgRNA sequence	GAGTCGGCAGATAATTTGGATGG
sgRNA2 sequence	GTACACAGAACTGTGATCGAAGG

 

left_200bp	right_200bp
ATCTTCCGTATTGATGACGAATTATCAATACAAATATATTACTGCAATAATTATTGAGTTTTTGTTTTACTTAATATAAATTTTGGAATAAATTCGTTAAAATTATTTTTAATTAAAAAAATTAAAATATCTTTGCACACTGCACGGGATGCAATTCGTTACGCGATTGTTTCTCGATTTTTAGGGTGACCAGTCCATCC	CGAAGGAGATGGTCCAAGCCATCCATTGTAGAAGGAAAGAGGCAAGACGGAAATGTGTTTCTTACATCTATATTTTATGCACGTGTCAATTACAATTCCAAAGGATACTTATAAAAAAAATGTAAAAATATTCATGTGCAACGCGACGCGGTTTGGCGGAGGTGATCTGGCGATAGTGAAGGAGCGGCGCCGCGCTGGGA

 

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