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CRIMIC CR71126

Gene CG4957

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	2L:10268872..10269904	31B1-31B1

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GTGGTCTTGCGTGCATTATGG	TTGCAGCGCGTACACAGATACT

Images

Construct Design Sequences

sgRNA sequence	CAAGCCCAGCAACCGATTCGTGG
sgRNA2 sequence	CATAGATATCCTCTAAGCCTGGG

 

left_200bp	right_200bp
AAATACCCCATTTAACTTAAATCAAATATATTTGAAAATTTAGACCACATTTTTTATAGCAACACGGATTTTTAGGAACTTCTGCGCCGGTAACACTAGTCCACTTTTTAGATCACAACACTAGAACTTAAAATGTTACGAAATGTGTGCACATTTTCCACGCATTTTGGAAAATGCATATTCCAAGCCCAGCAACCGAT	CTTAGAGGATATCTATGACAATGTGGAACAAAAATGACTTGTTGAATAAAACGTTGAGTTTAAACTTGAATAAATAAATTTGTTTCTAACTGATTAAGATACACTGTTAAGTAACAGAGTGTGCAATTTTCAGTGTTCCCGTTTCAGCGCTAAACGTTTCTGTTATTTTAAAGCTTATGTAAACTGGCTGGAATACCCTT

 

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