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CRIMIC CR71127

Gene CG13392

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	2L:8381821..8382807	29B3-29B4

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GTTGCAGGATTCGACGAGCACATT	ACTCACGTTTCAAACAGTAAATGC

Images

Construct Design Sequences

sgRNA sequence	TGTATTCAACATTCTCACACCGG
sgRNA2 sequence	TTGTTAACTAGCTCAAATAGAGG

 

left_200bp	right_200bp
GCATGTACTCGGTTCCTACGTTTGTGAGGCATTTTAAGCAGACTCGCAAGAATTATGGTAGTACACTTCAATTCGCCTCTGATATAAAACAGCACGCAACACTGATTTGACCATTTAACAGCACTGATATGTCATCATCTGTAAATTTCGCGTTACTTTCCGAGACCTTTTTCCGATACCCACTGTATTCAACATTCTCA	TAGAGGCGCATTTAAGAGCTTATTCTAAATGCATTTTTTAAAATTTGTGATTTTTTACATATATCTGGAGGGTTCTAGAGTGCAATTAATGTAAATTAAGCAAGTGGACTTAATTTTAAATATAAAAAAATTATTTTCTTCATTATGCCATCTCTAAATCTATCGATATCTCATCCAGTTTGCCGCATTGTCTATCGAGC

 

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