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CRIMIC CR71128

Gene Tspo

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	2L:559612..560523	21E2-21E2		Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CGGCTTAGCAATGACTTTGCAGCT	TTTTCAGAGCCCATTTTCGCC

Images

Construct Design Sequences

sgRNA sequence	CAGAGTTCGCACTGCAAATGAGG
sgRNA2 sequence	GCTTTCCGACCTATCGAACCAGG

 

left_200bp	right_200bp
AATTCTAGCGTCTTTGATTTCAAGTTGCATTCCACATACTTTAGCTAGTTTGAATCTAGCCAAATTGGCATCGCTGGAGCTTACCCACACAATCATCCGTCGCGTATTCGCCCCGTTAACACGCAATACTCGTGCATTCGAATGAAATCACCGGCAAACAAAACAGTGAAACCAGAAGGTTCGCCACTACTGCGCCTCAT	ACCAGGGCTGGGCAAAGTTGGATAAAATATCGATAAGCAGTGCCTCCAATGGGGCGCTAAATTCAAAGTATCATATGCTGCTGCACTTTCAAAAATTCCCTATGTTTTATGTGTTAATAATAAATTAAAGAATCGTAATTTTCAATGCTATTCTCGAGAACGATTAATAGGCTACAATTTGTTGTGACATTGCAATGGAA

 

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