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CRIMIC CR71129

Gene Ufsp1

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	2R:6974038..6975133	42E1-42E1

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CGGAATAGCCTTTCATCATGACTC	AGTAGCCCAGACTGAACTTGACCA

Images

Construct Design Sequences

sgRNA sequence	GGAGCAGGTGAATCAGGCCTAGG
sgRNA2 sequence	AATAGTAATCAAGAGCTGAGCGG

 

left_200bp	right_200bp
CGTTAATTTTATTTTCCGCATTATTGTTATTTTATTTTGGCTGACTTTGTCATTGGTGCATGACGATTCAGCTTGTACCATCTCTAGCCAGAGCAAAAAAAAAAACATTCCAATTTCTTTGCTGCGATTTCACTTTCACAAAACAAATTACGGCTGCCCAAGGTAAATAAACAAAAAAGAAAAGGAGCAGGTGAATCAGG	AGCTCTTGATTACTATTACTTACTGAAAGTTGCCTTATAGCCACGTTTCTCTTCCATTTTAGCGGCCGATACTCAATGGACCTTATCAATTATTAAATCGGACCGCACGATAATGGCGCTGATAAACCGGCGGCATTGAGCCCCAGAACCTCGACAAGCAGCTACTGCCAGCTGTTAAGCATATCAAACAGCGCGAACAT

 

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