CR71131

CRIMIC CR71131

Gene RN-tre

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	2R:13984815..13990707	50C23-50C2		Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TCACACTCACACAACGCAGATCAG	GTTGCCTTTCGCCTTTACATTCGTTT

Images

Construct Design Sequences

sgRNA sequence	AGGATTTGGCGGGTGGGGGAGGG
sgRNA2 sequence	AGGCGGCGGCCAACCGGAGCGGG

left_200bp	right_200bp
AGTGTTGTTATTGTGGATCGCTTTCGCATGTTAATGCTTTTGTGTGTTATTTTTTCCATTCCATTTTTTTTTTATCAAAGTTTCGCGCTATCGCCCAGTCAGCTCAGAAGCAGCCGCTCCCCAACTGAGAGCACAGAGAAACCAGCTCTGGCCACTTGCTCCTCCGGATCCGGCTCCTGCTCCTGTCCCCGATCCCCTCC	AGCGGGATCACCTAGTTCCTGGTGCTAGCCGTCGTCTGCCTTTGATTAGCCTTAAGTCTTCAAGCCCTGCCACCGCTGCAAACACTAGAGCTAGGCCTTCCATGGGTGCAGCCAATAGCAATCACCAAGCTTCGAGAGAATGAGAGCACACGCACACACACTTTGTACATACACCGCGGGAAGCTTGGGGCACGACTTGG