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CRIMIC CR71132

Gene CG14805

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	X:1877317..1879077	2B14-2B14

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CAAATTGATCAGCGTCAGGTGCAT	AGCAGCGCACAGACGACATTATGT

Images

Construct Design Sequences

sgRNA sequence	ATTTCAACCCGATCACAACATGG
sgRNA2 sequence	CGTAATTGAAAGCAAGCTTAAGG

 

left_200bp	right_200bp
CGAATTTGGATGTGACCAAGTTTCGCGAAGGGAAAAACCACATCGATTATTTTAGTAACTGGTCGATACGCCAAGACAAATCATGGTTTCGCTTATCGATAGGAAACTACCGCTTATTTCCGGTTGAACTTTAGAAGTCGTAATGCAATAACAATATTGGTACGTATTGTAACAACGTCGCGCATTTCAACCCGATCACA	GCTTGCTTTCAATTACGTTTACTTAGGACTTAGGACAAAAATACATGATAATAGAAAAAAAACAGCACTGATTGTGCCTAAAAACGATTTGGATGAACAAAACAATAACTTCTCGTAGAGGGATCTACTTGGGTGCCGCTTCGCTGCCCGCCTGCCCGTCCATGTTGTCATCTCCCGATTCGGGAGCGTTGTCATTTTCC

 

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