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CRIMIC CR71133

Gene CG1647

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	3R:28820221..28824508	98E5-98E5

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GCTTCGCCGTACGATGATTAACTTG	CCACGATTACGTATATGTATGTTGTCG

Images

Construct Design Sequences

sgRNA sequence	CTTTAATCATGCTAAATTACCGG
sgRNA2 sequence	CATGCCCTCCTCATTCTATTAGG

 

left_200bp	right_200bp
TTGGCTAAATATTTTTTTTAACGAGTTTCAAAAATTATACTTAAAATCAAATTTGCTAATTTTGAGATAAGCAAAAAATTCGATCCTATCGATAGCACATCGATTTCTACCAACCTTGTTAGCCGCTGACCAACACTAAGCGTATTGGAATAAACAAAATGCAAGTTCAACTGGAGATAAAACCTTTAATCATGCTAAAT	ATTAGGAGCTGTAAATATGTACGGAATGGTGAATTGTGCTTTTGTAATTGGATACGAGTAGAATTGGAGCTACCACGAATTGAGAATAAACTTCTTTCGGATAGCATTATTTTTAAGTTAGATATACACAACTTTATTACATAAATTAATGGCTTATATGTTAGCATATGTAAGTATAATGTATTTAAATACATACATCG

 

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