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CRIMIC CR71134

Gene CG8300

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p0	X:7104885..7106345	6F5-6F5

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CTGGAGGATTGGAACTGGCATC	GGCTAAATGAGTCACGCTAAATGG

Images

Construct Design Sequences

sgRNA sequence	CTACATGACCATGTACTAAATGG
sgRNA2 sequence

 

left_200bp	right_200bp
GACAATGTGAGCGCCCTTGAACTGCAGTCCAACAAGCGCATGCTGTACTTTAGCGACGGCGTCATGGAGGAGTTGTCCTCGGGCAGCGAGGACGAGGCGGATGCGGAAATGGGCGACAAATGCTACGACGTCCATTTAAACGAGGTACATACATTCAGCAAGGATTATGGGTGGAGTAATGCACTTCCGGCCATCCATTT	AGTACATGGTCATGTAGCTGCATTGCTTGTTTACCTTTTTTAAAGCGGGATTGTCCGGATTAACACAGTTAGTTAAATTTGCTCAAGATTAGCCCAGTTCTCCTGATCCAGATAATAACTTTTCACTTTGAGCTCGAGACGTGACTAATGTTCGAAGCGAATTATGATAATAATGTGAGAAATTTTATATCTTTCAATGA

 

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