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CRIMIC CR71135

Gene CG1663

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	2R:9836985..9838481	46B4-46B4

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GAATAAGACGTGCAACTACCTATC	CGGCGAGAATGTGGTTCTAGTTAG

Images

Construct Design Sequences

sgRNA sequence	GCAGCATTTGATTAAAATTGAGG
sgRNA2 sequence	CGAAGAAATGGAATCATATGAGG

 

left_200bp	right_200bp
GTCGAATTTTCCAACACTTGCGCTTGTAAATGAGAATACTTATCGGAAAACAAATCGTACCTGAAAACGATACTTAGTTCATAAAATGTGTAGTTTTCGGAATAAATTGCATGCAGATTCTAAGGACATGATACCAAAACACTCAAATAAGAAGCATTATGTAGAAAGTGCACGCGTATACATGAGAAGTGACTCCTCAA	ATGAGGAaGATGATCCAGAGGAATTGgACAGATTCGTTAGTTAAGTACGTTTCGAAAAATATAAAATAGTAGTTTAAATTTCTTTAAAAGTAAAACGATTTCAAACCTTAAATGGAAATATATCATATAACTTAATTTGTATACATTGTAGCGTAACTTAATTATATAcATCAATAATTTACATATATTCTTAGCTACTT

 

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