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CRIMIC CR71137

Gene CG4496

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	2L:7185461..7190010	27E4-27E4

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GTTCGTATGCGACTTGTCCAGGAT	GCTGTAGCTCATCATTCAGTTCTTGG

Images

Construct Design Sequences

sgRNA sequence	TTGTAACATTGCAGATTTGATGG
sgRNA2 sequence	GCGCGAGAGTTGTAAACTCTTGG

 

left_200bp	right_200bp
GGTTTTATTGTAATTATTCGATACTAGAAAGTACGCTTCTTGGAAATACTGATAAGTGCCGATTTATCCATAGTTGAATGTGATTCAAATAAAACACGCCTAAATGTATGCAATGTTTTTTTCTGATAGTGCGATGATAGTGCAATTCTTAGGTTCCTTTCTAAAACTTTTCTAAATTGTTTATTGTAACATTGCAGATT	TCTTGGTCATTCTTTTTAATGCATAAAGAGGATTGAAAATCCCATATTTCATATAAATTAGCTATTAAGGATCATTAAAACATTCCTTATTAATATATTCATATCTTTTTAAAGTGTTTTATATCACTATTAATTTATTCATTCATATTCTTTTTTAAGCACTTATAATACACTTTTGAAATGTAAAATGTAACGTGCGC

 

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